The Research on Sample-pretreatment of Organic Carbon and Stable Nitrogen Isotopes in Marine Sediments

CHEN Li-lei; ZHANG Yuan-yuan; HE Xing-liang; ZHANG Pei-yu; ZHU Zhi-gang; LI Xiao-lin

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Article Navigation > Acta Sedimentologica Sinica > 2014 > 32(6): 1046-1051

CHEN Li-lei, ZHANG Yuan-yuan, HE Xing-liang, ZHANG Pei-yu, ZHU Zhi-gang, LI Xiao-lin. The Research on Sample-pretreatment of Organic Carbon and Stable Nitrogen Isotopes in Marine Sediments[J]. Acta Sedimentologica Sinica, 2014, 32(6): 1046-1051.

Citation:

CHEN Li-lei, ZHANG Yuan-yuan, HE Xing-liang, ZHANG Pei-yu, ZHU Zhi-gang, LI Xiao-lin. The Research on Sample-pretreatment of Organic Carbon and Stable Nitrogen Isotopes in Marine Sediments[J]. Acta Sedimentologica Sinica, 2014, 32(6): 1046-1051.

The Research on Sample-pretreatment of Organic Carbon and Stable Nitrogen Isotopes in Marine Sediments

1.
Key Laboratory of Marine Hydrocarbon Resources and Environmental Geology, Ministry of Land and Resources, Qingdao, Shandong 266071;
2.
Qingdao Institute of Marine Geology, Qingdao, Shandong 266071;
3.
Department of Environmental Science and Engineering, Qingdao University, Qingdao, Shandong 266071

Received Date: 2013-09-03
Rev Recd Date: 2014-09-25
Publish Date: 2014-12-10

Abstract

The organic carbon and nitrogen isotopes in marine sediments provide a lot of information about marine paleoenvironmental and carbon and nitrogen cycles. However, different pretreatment methods have different impacts on the accuracy of the results of organic carbon and nitrogen isotope. This paper analyzed the factors affecting the pretreatment process on the basis of a great many condition tests. (1) Acid treatment Containers Material: The organic carbon and nitrogen isotope values of acid treatment with polypropylene and glass centrifugal tubes make no difference. (2) Drying methods: The sample δ¹³C will be fractionationed for the escape of light carbon and volatile components caused by heat-drying. The sample δ¹⁵N will be fractionationed caused by the fresh and acid-treated samples heat-dried. (3) The drive acid method and wash acid times: Dissolved organic carbon and nitrogen will be loss caused by washing, resulting in δ¹³C and δ¹⁵N values more partial than the true ones. Meanwhile, steaming acid can not get rid of chloride ions effectively. The effect of acid wash times on δ¹³C is very small, but it will result in δ¹⁵N partial. In short, using of freeze-drying, the choice of PP centrifugal tube as the main acid treatment washing and drying vessels, with good reproducibility, get more satisfactory and accurate test δ¹³C and δ¹⁵N values.
- marine sediment,
- organic carbon and stable nitrogen isotopes,
- condition test

References

[1]	Lamb A L, Vane C H, Wilson G P,et al. Assessing δ¹³C and C/N ratios from organic material in archived cores as Holocene sea level and palaeoenvironmental indicators in the Humber Estuary, UK[J]. Marine Geology, 2007, 244(1): 109-128
[2]	Mackie E A, Lloyd J M, Leng M J,et al. Assessment of δ¹³C and C/N ratios in bulk organic matter as palaeosalinity indicators in Holocene and Late glacial isolation basin sediments, northwest Scotland[J]. Journal of Quaternary Science, 2007, 22(6): 579-591
[3]	Wilson G P, Lamb A L, Leng M J,et al. δ¹³C and C/N as potential coastal palaeoenvironmental indicators in the Mersey Estuary, UK[J]. Quaternary Science Reviews, 2005, 24(18): 2015-2029
[4]	Sharp Z. Principles of stable isotope geochemistry.Upper Saddle River, NJ, USA: Pearson Education, 2007
[5]	Mampuku M, Yamanaka T, Uchida M,et al. Changes in C₃/C₄ vegetation in the continental interior of the Central Himalayas associated with monsoonal paleoclimatic changes during the last 600 kyr[J]. Climate of the Past, 2008, 4(1): 1-9
[6]	Scholz C A, Talbot M R, Brown E T,et al. Lithostratigraphy, physical properties and organic matter variability in Lake Malawi Drillcore sediments over the past 145,000 years[J]. Palaeogeography, Palaeoclimatology, Palaeoecology, 2011, 303(1): 38-50
[7]	Brodie C R, Leng M J, Casford J S L,et al. Evidence for bias on C and N concentrations and δ¹³C composition of terrestrial and aquatic organic materials due to pre-analysis acid preparation methods[J]. Chemical Geology, 2011, 282(3): 67-83
[8]	葛晨东,王颖,Pedersen T F,等. 海南岛万泉河口沉积物有机碳、氮同位素的特征及其环境意义[J]. 第四纪研究,2007,27(5):845-852[Ge Chendong, Wang Ying, Pedersen T F, et al. Variability of organic carbon isotope, nitrogen isotope, and C/N in the Wanquan river estuary, eastern Hainan island, China and its environmental implications[J]. Quaternary Sciences, 2007, 27(5): 845-852]
[9]	Brodie C R, Casford J S, Lloyd J M,et al. Evidence for bias in C/N, δ¹³C and δ¹⁵N values of bulk organic matter, and on environmental interpretation, from a lake sedimentary sequence by pre-analysis acid treatment methods[J]. Quaternary Science Reviews, 2011, 30(21): 3076-3087
[10]	King P, Kennedy H, Newton P P,et al. Analysis of total and organic carbon and total nitrogen in settling oceanic particles and a marine sediment: an interlaboratory comparison[J]. Marine Chemistry, 1998, 60(3): 203-216
[11]	Schubert C J, Nielsen B. Effects of decarbonation treatments on δ¹³C values in marine sediments[J]. Marine Chemistry, 2000, 72(1): 55-59
[12]	王金权. 样品预处理对有机碳同位素分析结果的影响[J]. 古生物学报,2005,44(3):472-477 [Wang Jinquan. Sample-pretreatment effects on analytical results of organic C-isotopes[J]. Acta Palaeontologica Sinica, 2005, 44(3): 472-477]
[13]	吴夏,黄俊华,白晓,等. 沉积岩总有机质碳同位素分析的前处理影响[J]. 地球学报,2008,29(6):677-683 [Wu Xia, Huang Junhua, Bai Xiao, et al. Sample-pretreatment effects on analytical results of total organic carbon isotopes in sedimentary rocks[J]. Acta Geoscientica Sinica, 2008, 29(6): 677-683]
[14]	曹蕴宁,刘卫国,宁有丰,等. 土壤有机碳同位素样品制备过程的影响因素讨论[J]. 地球化学,2005,34(4):395-403 [Cao Yunning, Liu Weiguo, Ning Youfeng, et al. Effects of soil sample preparation process on δ¹³C of organic matter[J]. Geochimica, 2005, 34(4): 395-403]
[15]	卢凤艳,安芷生. 鹤庆钻孔沉积物总有机碳、氮含量测定的前处理方法及其环境意义[J]. 地质力学学报,2010,16(4):393-401[Lu Fengyan, An Zhisheng. Pretreatment methods for analyzing the total organic carbon and nitrogen contents of Heqing core sediments and their environmental significances[J]. Journal of Geomechanics, 2010, 16(4): 393-401]
[16]	张媛媛,贺行良,孙书文,等. 元素分析仪—同位素比值质谱仪测定海洋沉积物有机碳稳定同位素方法初探[J]. 岩矿测试,2012,31(4):627-631[Zhang Yuanyuan, He Xingliang, Sun Shuwen, et al. A preliminary study on the determination of organic carbon stable isotope of marine sediment by element analyzer-isotope ratio mass spectrometer[J]. Rock and Mineral Analysis, 2012, 31(4): 627-631]
[17]	迟云平,张洒,吴松,等. 不同实验条件对早新生代沉积物有机碳同位素的影响[J]. 地球学报,2012,33(6):967-976[Chi Yunping, Zhang Sa, Wu Song, et al. The influences of different experimental conditions on organic carbon isotopes of Early Cenozoic sediments[J]. Acta Geoscientica Sinica, 2012, 33(6): 967-976]
[18]	于雯泉,钟少军. 海洋沉积物有机碳分析方法中干燥预处理过程人为误差的发现及其意义[J]. 环境科学学报,2007,27(5):861-867[Yu Wenquan, Zhong Shaojun. Freeze-drying pretreatment improves organic carbon determinations of marine sediments[J]. Acta Scientiae Circumstantiae, 2007, 27(5): 861-867]
[19]	王浩然,陈业材. 湖泊沉积物有机碳分析的最优化方法[J]. 贵州环保科技,1996,2(4):21-24[Wang Haoran, Chen Yecai. Lake sediment organic carbon analysis, optimization methods[J]. Guizhou Environmental Science and Technology, 1996, 2(4): 21-24]
[20]	Fry B, Scalan R S, Parker P L. Stable carbon isotope evidence for two sources of organic matter in coastal sediments: seagrasses and plankton[J]. Geochimica et Cosmochimica Acta, 1977, 41(12): 1875-1877

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

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The Research on Sample-pretreatment of Organic Carbon and Stable Nitrogen Isotopes in Marine Sediments

1. Key Laboratory of Marine Hydrocarbon Resources and Environmental Geology, Ministry of Land and Resources, Qingdao, Shandong 266071;

2. Qingdao Institute of Marine Geology, Qingdao, Shandong 266071;

3. Department of Environmental Science and Engineering, Qingdao University, Qingdao, Shandong 266071

Received Date: 2013-09-03

Rev Recd Date: 2014-09-25

Publish Date: 2014-12-10

Key words:

marine sediment /
organic carbon and stable nitrogen isotopes /
condition test

Abstract: The organic carbon and nitrogen isotopes in marine sediments provide a lot of information about marine paleoenvironmental and carbon and nitrogen cycles. However, different pretreatment methods have different impacts on the accuracy of the results of organic carbon and nitrogen isotope. This paper analyzed the factors affecting the pretreatment process on the basis of a great many condition tests. (1) Acid treatment Containers Material: The organic carbon and nitrogen isotope values of acid treatment with polypropylene and glass centrifugal tubes make no difference. (2) Drying methods: The sample δ¹³C will be fractionationed for the escape of light carbon and volatile components caused by heat-drying. The sample δ¹⁵N will be fractionationed caused by the fresh and acid-treated samples heat-dried. (3) The drive acid method and wash acid times: Dissolved organic carbon and nitrogen will be loss caused by washing, resulting in δ¹³C and δ¹⁵N values more partial than the true ones. Meanwhile, steaming acid can not get rid of chloride ions effectively. The effect of acid wash times on δ¹³C is very small, but it will result in δ¹⁵N partial. In short, using of freeze-drying, the choice of PP centrifugal tube as the main acid treatment washing and drying vessels, with good reproducibility, get more satisfactory and accurate test δ¹³C and δ¹⁵N values.

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Reference (20)

[1]	Lamb A L, Vane C H, Wilson G P,et al. Assessing δ¹³C and C/N ratios from organic material in archived cores as Holocene sea level and palaeoenvironmental indicators in the Humber Estuary, UK[J]. Marine Geology, 2007, 244(1): 109-128
[2]	Mackie E A, Lloyd J M, Leng M J,et al. Assessment of δ¹³C and C/N ratios in bulk organic matter as palaeosalinity indicators in Holocene and Late glacial isolation basin sediments, northwest Scotland[J]. Journal of Quaternary Science, 2007, 22(6): 579-591
[3]	Wilson G P, Lamb A L, Leng M J,et al. δ¹³C and C/N as potential coastal palaeoenvironmental indicators in the Mersey Estuary, UK[J]. Quaternary Science Reviews, 2005, 24(18): 2015-2029
[4]	Sharp Z. Principles of stable isotope geochemistry.Upper Saddle River, NJ, USA: Pearson Education, 2007
[5]	Mampuku M, Yamanaka T, Uchida M,et al. Changes in C₃/C₄ vegetation in the continental interior of the Central Himalayas associated with monsoonal paleoclimatic changes during the last 600 kyr[J]. Climate of the Past, 2008, 4(1): 1-9
[6]	Scholz C A, Talbot M R, Brown E T,et al. Lithostratigraphy, physical properties and organic matter variability in Lake Malawi Drillcore sediments over the past 145,000 years[J]. Palaeogeography, Palaeoclimatology, Palaeoecology, 2011, 303(1): 38-50
[7]	Brodie C R, Leng M J, Casford J S L,et al. Evidence for bias on C and N concentrations and δ¹³C composition of terrestrial and aquatic organic materials due to pre-analysis acid preparation methods[J]. Chemical Geology, 2011, 282(3): 67-83
[8]	葛晨东,王颖,Pedersen T F,等. 海南岛万泉河口沉积物有机碳、氮同位素的特征及其环境意义[J]. 第四纪研究,2007,27(5):845-852[Ge Chendong, Wang Ying, Pedersen T F, et al. Variability of organic carbon isotope, nitrogen isotope, and C/N in the Wanquan river estuary, eastern Hainan island, China and its environmental implications[J]. Quaternary Sciences, 2007, 27(5): 845-852]
[9]	Brodie C R, Casford J S, Lloyd J M,et al. Evidence for bias in C/N, δ¹³C and δ¹⁵N values of bulk organic matter, and on environmental interpretation, from a lake sedimentary sequence by pre-analysis acid treatment methods[J]. Quaternary Science Reviews, 2011, 30(21): 3076-3087
[10]	King P, Kennedy H, Newton P P,et al. Analysis of total and organic carbon and total nitrogen in settling oceanic particles and a marine sediment: an interlaboratory comparison[J]. Marine Chemistry, 1998, 60(3): 203-216
[11]	Schubert C J, Nielsen B. Effects of decarbonation treatments on δ¹³C values in marine sediments[J]. Marine Chemistry, 2000, 72(1): 55-59
[12]	王金权. 样品预处理对有机碳同位素分析结果的影响[J]. 古生物学报,2005,44(3):472-477 [Wang Jinquan. Sample-pretreatment effects on analytical results of organic C-isotopes[J]. Acta Palaeontologica Sinica, 2005, 44(3): 472-477]
[13]	吴夏,黄俊华,白晓,等. 沉积岩总有机质碳同位素分析的前处理影响[J]. 地球学报,2008,29(6):677-683 [Wu Xia, Huang Junhua, Bai Xiao, et al. Sample-pretreatment effects on analytical results of total organic carbon isotopes in sedimentary rocks[J]. Acta Geoscientica Sinica, 2008, 29(6): 677-683]
[14]	曹蕴宁,刘卫国,宁有丰,等. 土壤有机碳同位素样品制备过程的影响因素讨论[J]. 地球化学,2005,34(4):395-403 [Cao Yunning, Liu Weiguo, Ning Youfeng, et al. Effects of soil sample preparation process on δ¹³C of organic matter[J]. Geochimica, 2005, 34(4): 395-403]
[15]	卢凤艳,安芷生. 鹤庆钻孔沉积物总有机碳、氮含量测定的前处理方法及其环境意义[J]. 地质力学学报,2010,16(4):393-401[Lu Fengyan, An Zhisheng. Pretreatment methods for analyzing the total organic carbon and nitrogen contents of Heqing core sediments and their environmental significances[J]. Journal of Geomechanics, 2010, 16(4): 393-401]
[16]	张媛媛,贺行良,孙书文,等. 元素分析仪—同位素比值质谱仪测定海洋沉积物有机碳稳定同位素方法初探[J]. 岩矿测试,2012,31(4):627-631[Zhang Yuanyuan, He Xingliang, Sun Shuwen, et al. A preliminary study on the determination of organic carbon stable isotope of marine sediment by element analyzer-isotope ratio mass spectrometer[J]. Rock and Mineral Analysis, 2012, 31(4): 627-631]
[17]	迟云平,张洒,吴松,等. 不同实验条件对早新生代沉积物有机碳同位素的影响[J]. 地球学报,2012,33(6):967-976[Chi Yunping, Zhang Sa, Wu Song, et al. The influences of different experimental conditions on organic carbon isotopes of Early Cenozoic sediments[J]. Acta Geoscientica Sinica, 2012, 33(6): 967-976]
[18]	于雯泉,钟少军. 海洋沉积物有机碳分析方法中干燥预处理过程人为误差的发现及其意义[J]. 环境科学学报,2007,27(5):861-867[Yu Wenquan, Zhong Shaojun. Freeze-drying pretreatment improves organic carbon determinations of marine sediments[J]. Acta Scientiae Circumstantiae, 2007, 27(5): 861-867]
[19]	王浩然,陈业材. 湖泊沉积物有机碳分析的最优化方法[J]. 贵州环保科技,1996,2(4):21-24[Wang Haoran, Chen Yecai. Lake sediment organic carbon analysis, optimization methods[J]. Guizhou Environmental Science and Technology, 1996, 2(4): 21-24]
[20]	Fry B, Scalan R S, Parker P L. Stable carbon isotope evidence for two sources of organic matter in coastal sediments: seagrasses and plankton[J]. Geochimica et Cosmochimica Acta, 1977, 41(12): 1875-1877

The Research on Sample-pretreatment of Organic Carbon and Stable Nitrogen Isotopes in Marine Sediments

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通讯作者: 陈斌, bchen63@163.com

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